Chromatin modifications are associated with many aspects of gene expression, yet their role
in cellular transitions during development remains elusive. Here, we use a new approach to …

Abstract Development requires the establishment of precise patterns of gene expression,
which are primarily controlled by transcription factors binding to cis-regulatory modules. …

By the onset of morphogenesis, Drosophila embryos consist of about 6000 cells that express
distinct gene combinations. Here, we used single-cell sequencing of precisely staged …

Genetic studies have identified numerous sequence-specific transcription factors that control
development, yet little is known about their in vivo distribution across animal genomes. We …

Metabolic reprogramming is a key feature of many cancers, but how and when it contributes
to tumorigenesis remains unclear. Here we demonstrate that metabolic reprogramming …

The early Drosophila embryo is emerging as a premiere model system for the computational
analysis of gene regulation in development because most of the genes, and many of the …

This protocol describes the batch isolation of tissue-specific chromatin for immunoprecipitation
(BiTS-ChIP) for analysis of histone modifications, transcription factor binding, or …

The transcription factors of the Snail family are key regulators of epithelial–mesenchymal
transitions, cell morphogenesis, and tumor metastasis. Since its discovery in Drosophila ∼25 …

In response to fasting or hyperglycemia, the pancreatic β-cell alters its output of secreted
insulin; however, the pathways governing this adaptive response are not entirely established. …

The dorsal-ventral patterning of the Drosophila embryo is controlled by a well-defined gene
regulation network. We wish to understand how changes in this network produce …