Transgene Expression of Green Fluorescent Protein and Germ Line Transmission in Cloned Pigs Derived from In Vitro Transfected Adult Fibroblasts

…, A Perota, I Lagutina, S Colleoni, R Duchi… - Cloning and stem …, 2008 - liebertpub.com
The pig represents the xenogeneic donor of choice for future organ transplantation in humans
for anatomical and physiological reasons. However, to bypass several immunological …

[HTML][HTML] A cloned horse born to its dam twin

…, G Crotti, S Colleoni, P Turini, N Ponderato, R Duchi… - Nature, 2003 - nature.com
Several animal species, including sheep, mice, cattle, goats, rabbits, cats, pigs and, more
recently, mules 1 have been reproduced by somatic cell cloning, with the offspring being a …

Cellular and molecular deviations in bovine in vitro-produced embryos are related to the large offspring syndrome

…, C Wrenzycki, D Herrmann, R Duchi… - Biology of …, 2002 - academic.oup.com
The large offspring syndrome (LOS) is observed in bovine and ovine offspring following
transfer of in vitro-produced (IVP) or cloned embryos and is characterized by a multitude of …

Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses: from the research laboratory to clinical practice

C Galli, R Duchi, S Colleoni, I Lagutina, G Lazzari - Theriogenology, 2014 - Elsevier
Assisted reproductive techniques developed for cattle in the last 25 years, like ovum pick up
(OPU), intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transfer, have been …

[HTML][HTML] Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type

I Lagutina, G Lazzari, R Duchi, S Colleoni… - …, 2005 - rep.bioscientifica.com
The objective of the present work was to investigate and clarify the factors affecting the
efficiency of somatic cell nuclear transfer (NT) in the horse, including embryo reconstruction, in …

Comparative aspects of somatic cell nuclear transfer with conventional and zona-free method in cattle, horse, pig and sheep

I Lagutina, G Lazzari, R Duchi, P Turini, I Tessaro… - Theriogenology, 2007 - Elsevier
Nuclear transfer (NT) is a complex procedure that requires considerable technical skills.
Over the years attempts have been made to simplify the micromanipulations involved and to …

Laboratory production of equine embryos

…, L Landriscina, G Dolci, M Benedetti, R Duchi… - Journal of equine …, 2020 - Elsevier
Assisted reproduction technologies (ART) are well developed in humans and cattle and are
gaining momentum also in the equine industry because of the fact that the mare does not …

Mammalian leukocytes contain all the genetic information necessary for the development of a new individual

C Galli, R Duchi, RM Moor, G Lazzari - Cloning, 1999 - liebertpub.com
We have used leukocytes and oocytes from commercially slaughtered animals to clone a
progeny tested Brown Swiss bull. Mononuclear cells were separated from the heparinized …

Developmental potential of bovine androgenetic and parthenogenetic embryos: a comparative study

I Lagutina, G Lazzari, R Duchi, C Galli - Biology of reproduction, 2004 - academic.oup.com
In this study, we compared the developmental capacity of bovine haploid and diploid
androgenetic and parthenogenetic embryos obtained by different methods. Androgenetic embryos …

Biomimetic glyconanoparticle vaccine for cancer immunotherapy

EM Reuven, S Leviatan Ben-Arye, H Yu, R Duchi… - ACS …, 2019 - ACS Publications
Cancer immunotherapy aims to harness the immune system to combat malignant processes.
Transformed cells harbor diverse modifications that lead to formation of neoantigens, …