Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples

…, K Gangavarapu, G Oliveira, R Robles-Sikisaka… - Nature protocols, 2017 - nature.com
Genome sequencing has become a powerful tool for studying emerging infectious diseases;
however, genome sequencing directly from clinical samples (ie, without isolation and culture…

[HTML][HTML] Wastewater sequencing reveals early cryptic SARS-CoV-2 variant transmission

…, E Parker, SA Perkins, KS Ramesh, R Robles-Sikisaka… - Nature, 2022 - nature.com
As SARS-CoV-2 continues to spread and evolve, detecting emerging variants early is critical
for public health interventions. Inferring lineage prevalence by clinical testing is infeasible …

[PDF][PDF] Emergence and rapid transmission of SARS-CoV-2 B. 1.1. 7 in the United States

…, X Wang, D Wong, E Spencer, R Robles-Sikisaka… - Cell, 2021 - cell.com
The highly transmissible B.1.1.7 variant of SARS-CoV-2, first identified in the United Kingdom,
has gained a foothold across the world. Using S gene target failure (SGTF) and SARS-CoV…

Zika virus evolution and spread in the Americas

…, ND Grubaugh, G Oliveira, R Robles-Sikisaka… - Nature, 2017 - nature.com
Although the recent Zika virus (ZIKV) epidemic in the Americas and its link to birth defects
have attracted a great deal of attention 1 , 2 , much remains unknown about ZIKV disease …

Genomic epidemiology reveals multiple introductions of Zika virus into the United States

…, D Reyes, AM Bingham, LM Paul, R Robles-Sikisaka… - Nature, 2017 - nature.com
Zika virus (ZIKV) is causing an unprecedented epidemic linked to severe congenital abnormalities
1 , 2 . In July 2016, mosquito-borne ZIKV transmission was reported in the continental …

Altered oral viral ecology in association with periodontal disease

M Ly, SR Abeles, TK Boehm, R Robles-Sikisaka… - MBio, 2014 - Am Soc Microbiol
The human oral cavity is home to a large and diverse community of viruses that have yet to
be characterized in patients with periodontal disease. We recruited and sampled saliva and …

Human oral viruses are personal, persistent and gender-consistent

SR Abeles, R Robles-Sikisaka, M Ly, AG Lum… - The ISME …, 2014 - academic.oup.com
Viruses are the most abundant members of the human oral microbiome, yet relatively little is
known about their biodiversity in humans. To improve our understanding of the DNA viruses …

[HTML][HTML] Genomic epidemiology identifies emergence and rapid transmission of SARS-CoV-2 B. 1.1. 7 in the United States

…, X Wang, D Wong, E Spencer, R Robles-Sikisaka… - MedRxiv, 2021 - ncbi.nlm.nih.gov
As of January of 2021, the highly transmissible B. 1.1. 7 variant of SARS-CoV-2, which was
first identified in the United Kingdom (UK), has gained a strong foothold across the world. …

Nonsynonymous substitution in abalone sperm fertilization genes exceeds substitution in introns and mitochondrial DNA

EC Metz, R Robles-Sikisaka… - Proceedings of the …, 1998 - National Acad Sciences
Strong positive Darwinian selection acts on two sperm fertilization proteins, lysin and 18-kDa
protein, from abalone (Haliotis). To understand the phylogenetic context for this dramatic …

Association between living environment and human oral viral ecology

R Robles-Sikisaka, M Ly, T Boehm, M Naidu… - The ISME …, 2013 - academic.oup.com
The human oral cavity has an indigenous microbiota known to include a robust community
of viruses. Very little is known about how oral viruses are spread throughout the environment …