The Transgenic RNAi Project (TRiP), a Drosophila melanogaster functional genomics platform
at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-…

Being relatively simple and practical, Drosophila transgenic RNAi is the technique of top
priority choice to quickly study genes with pleiotropic functions. However, drawbacks have …

CRISPR/Cas9-based transcriptional activation (CRISPRa) has recently emerged as a
powerful and scalable technique for systematic overexpression genetic analysis in Drosophila …

In the Drosophila ovary, escort cells (ECs) extrinsically control germline stem cell (GSC)
maintenance and progeny differentiation. However, the underlying mechanisms remain poorly …

The flySAM/CRISPRa system has recently emerged as a powerful tool for gain-of-function
studies in Drosophila melanogaster. This system includes Gal4/UAS-driven dCas9 activators …

Notch signaling and epigenetic factors are known to play critical roles in regulating tissue
homeostasis in most multicellular organisms, but how Notch signaling coordinates with …

Much of our knowledge about the mechanisms underlying biological processes relies on
genetic approaches, whereby gene activity is reduced and the phenotypic consequences of …

Background: Cancer stem cells (CSC) carry out a vital responsibility throughout the entire
progress of colorectal cancer (CRC), and fulfil an essential biological function. However, …

The accurate calculation of the gas content of coalbed bed methane (CBM) reservoirs is of
great significance. However, due to the weak correlation between the logging response of …

The elimination of 2,3,6-trichloroanisole (2,3,6-TCA), which produces a musty-earthy off-odor
in water, by an ultraviolet (UV)/H 2 O 2 process was assessed. The removal of 88.1% of 2,3,…