Identification of host genes essential for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
infection may reveal novel therapeutic targets and inform our understanding of …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi),
and activation (CRISPRa) has enabled the systematic interrogation of gene function. …

Understanding the functional consequences of single-nucleotide variants is critical to
uncovering the genetic underpinnings of diseases, but technologies to characterize variants are …

Identification of host determinants of coronavirus infection informs mechanisms of viral
pathogenesis and can provide new drug targets. Here we demonstrate that mammalian SWItch/…

A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and
flexible manipulation of genomes in both targeted and large-scale experiments. Numerous …

Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic perturbations
because they can process multiple guide RNAs expressed as a single transcript, and …

Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for
understanding gene function. Generating such pairs of mammalian cells, however, is labor-…

Identification of host genes essential for SARS-CoV-2 infection may reveal novel therapeutic
targets and inform our understanding of COVID-19 pathogenesis. Here we performed a …

Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported
to expand the utility of CRISPR technology. Here, we assess the activity and specificity of …

Identifying host genes essential for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
has the potential to reveal novel drug targets and further our understanding of …