[PDF][PDF] Two RNase H2 mutants with differential rNMP processing activity reveal a threshold of ribonucleotide tolerance for embryonic development
R Uehara, SM Cerritelli, N Hasin, K Sakhuja, M London… - Cell reports, 2018 - cell.com
RNase H2 has two distinct functions: initiation of the ribonucleotide excision repair (RER)
pathway by cleaving ribonucleotides (rNMPs) incorporated during DNA replication and …
pathway by cleaving ribonucleotides (rNMPs) incorporated during DNA replication and …
MEK/ERK‐mediated oncogenic signals promote secretion of extracellular vesicles by controlling lysosome function
T Hikita, R Uehara, RE Itoh, F Mitani, M Miyata… - Cancer …, 2022 - Wiley Online Library
Cancer cells secrete large amounts of extracellular vesicles (EVs) originating from multivesicular
bodies (MVBs). Mature MVBs fuse either with the plasma membrane for release as EVs…
bodies (MVBs). Mature MVBs fuse either with the plasma membrane for release as EVs…
[HTML][HTML] Development of wearable sheet-type shear force sensor and measurement system that is insusceptible to temperature and pressure
…, S Shirogane, T Nakamura, T Umino, R Uehara… - Sensors, 2017 - mdpi.com
A sheet-type shear force sensor and a measurement system for the sensor were developed.
The sensor has an original structure where a liquid electrolyte is filled in a space composed …
The sensor has an original structure where a liquid electrolyte is filled in a space composed …
[HTML][HTML] Unlike the Escherichia coli counterpart, archaeal RNase HII cannot process ribose monophosphate abasic sites and oxidized ribonucleotides embedded in …
…, S Balachander, KD Koh, G Newnam, R Uehara… - Journal of Biological …, 2019 - ASBMB
The presence of ribonucleoside monophosphates (rNMPs) in nuclear DNA decreases
genome stability. To ensure survival despite rNMP insertions, cells have evolved a complex …
genome stability. To ensure survival despite rNMP insertions, cells have evolved a complex …
Crystal structures of the cell-division protein FtsZ from Klebsiella pneumoniae and Escherichia coli
…, M Ozawa, N Kuroda, S Tanaka, R Uehara… - … Section F: Structural …, 2020 - scripts.iucr.org
FtsZ, a tubulin-like GTPase, is essential for bacterial cell division. In the presence of GTP, FtsZ
polymerizes into filamentous structures, which are key to generating force in cell division. …
polymerizes into filamentous structures, which are key to generating force in cell division. …
Requirement of Ca2+ Ions for the Hyperthermostability of Tk-Subtilisin from Thermococcus kodakarensis
R Uehara, Y Takeuchi, S Tanaka, K Takano… - Biochemistry, 2012 - ACS Publications
Tk-subtilisin, a hyperthermostable subtilisin-like serine protease from Thermococcus
kodakarensis, matures from the inactive precursor, Pro-Tk-subtilisin (Pro-TKS), upon …
kodakarensis, matures from the inactive precursor, Pro-Tk-subtilisin (Pro-TKS), upon …
Formation of the high-affinity calcium binding site in pro-subtilisin E with the insertion sequence IS1 of pro-Tk-subtilisin
R Uehara, C Angkawidjaja, Y Koga, S Kanaya - Biochemistry, 2013 - ACS Publications
Subtilisin E is activated from its inactive precursor Pro-subtilisin E by autoprocessing and
degradation of the propeptide. Subtilisin E has two calcium binding sites, the high-affinity Ca1 …
degradation of the propeptide. Subtilisin E has two calcium binding sites, the high-affinity Ca1 …
[HTML][HTML] Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer
SK Deasy, R Uehara, SK Vodnala, HH Yang… - PLoS …, 2019 - journals.plos.org
Breast cancer is the second leading cause of cancer-related deaths in the United States, with
the majority of these deaths due to metastatic lesions rather than the primary tumor. Thus, a …
the majority of these deaths due to metastatic lesions rather than the primary tumor. Thus, a …
[HTML][HTML] Asteltoxin inhibits extracellular vesicle production through AMPK/mTOR-mediated activation of lysosome function
F Mitani, J Lin, T Sakamoto, R Uehara, T Hikita… - Scientific Reports, 2022 - nature.com
Cancer cells secrete aberrantly large amounts of extracellular vesicles (EVs) including exosomes,
which originate from multivesicular bodies (MVBs). Because EVs potentially contribute …
which originate from multivesicular bodies (MVBs). Because EVs potentially contribute …
Accelerated maturation of Tk‐subtilisin by a L eu→ P romutation at the C‐terminus of the propeptide, which reduces the binding of the propeptide to T k‐subtilisin
R Uehara, Y Ueda, DJ You, Y Koga… - The FEBS …, 2013 - Wiley Online Library
T k‐subtilisin, a subtilisin homologue (Gly70‐Gly398) from T hermococcus kodakarensis, is
matured from its precursor, P ro‐ T k‐subtilisin [ T k‐subtilisin in a pro form ( G ly1‐ G ly398)], …
matured from its precursor, P ro‐ T k‐subtilisin [ T k‐subtilisin in a pro form ( G ly1‐ G ly398)], …