… Samples were initially denatured at 95 C for 5 min, followed by 35 cycles of 94 C for 50 s,
40 C for 30 s, and 72 C for 60 s, with a final extension step at 72 C for 5 min [41]. PCR …

Structural changes in the gut microbial community have been shown to accompany the
progressive development of colorectal cancer. In this review we discuss recent hypotheses on …

… the microbiota adaptation to the host’s age-specific physiological and nutritional demands.
For … impact on human’s chances to attain “healthy ageing” and, consequently, reach longevity. …

… rate of 20 C s −1 : denaturation at 95 C for 15 s, annealing at 63 C for 20 s, extension at
72 C for 30 s and fluorescence acquisition at 90 C for 5 s; and (iii) melting curve analysis. …

… Twenty-four semi-supercentenarians (105+), ie 105-109 years old (18 females, 6 males,
mean age 106.2), defined “group S”, were enrolled for this study in Emilia Romagna and …

Objective Ageing is accompanied by deterioration of multiple bodily functions and
inflammation, which collectively contribute to frailty. We and others have shown that frailty co-varies …

Through human microbiome sequencing, we can better understand how host evolutionary
and ontogenetic history is reflected in the microbial function. However, there has been no …

Age-related alterations in human gut microbiota composition have been thoroughly described,
but a detailed functional description of the intestinal bacterial coding capacity is still …

… s, annealing at 55C for 30 s, then extension at 72C for 30 s, and the last extension step at
72C for 5 min. The Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA) …

… Thermal cycle consisted of an initial denaturation at 95C for 3 min, twentyfive cycles of
denaturation at 95C for 30s, annealing at 55C for 30 s, extension at 72C for 30 s and a final …