DNA methylation is a fundamentally important epigenetic modification of the mammalian
genome that has widespread influences on gene expression. During germ-cell specification …

Emerging single-cell epigenomic methods are being developed with the exciting potential
to transform our knowledge of gene regulation. Here we review available techniques and …

We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately
measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in …

Elucidating how and to what extent CpG islands (CGIs) are methylated in germ cells is
essential to understand genomic imprinting and epigenetic reprogramming 1 , 2 , 3 . Here we …

… Shown sa joint hierarchical clustering from 61 serum and 16 2i cells profiled using scM&T-seq,
as well as 20 serum and 12 2i ESCs profiled by scBS-seq (Smallwood et al. 2014), as …

Genomic imprinting requires the differential marking by DNA methylation of genes in male and
female gametes. In the female germline, acquisition of methylation imprint marks depends …

DNA methylation (DNAme) is an important epigenetic mark in diverse species. Our current
understanding of DNAme is based on measurements from bulk cell samples, which obscures …

Background Previously, a role was demonstrated for transcription in the acquisition of DNA
methylation at imprinted control regions in oocytes. Definition of the oocyte DNA methylome …

Erasure and subsequent reinstatement of DNA methylation in the germline, especially at
imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals. The mechanisms …

DNA methylation is considered a stable epigenetic mark, yet methylation patterns can vary
during differentiation and in diseases such as cancer. Local levels of DNA methylation result …