… the shells by demineralization with a recently developed ion exchange resin technique (S. …
We thank S. Brande for providing some of the shell material. SW is the incumbent of the 1. …

The design of new enzymes for reactions not catalysed by naturally occurring biocatalysts is
a challenge for protein engineering and is a critical test of our understanding of enzyme …

Many biologically formed calcite crystals contain intracrystalline macromolecules. The ways
in which they interact with growing calcite crystals were evaluated by monitoring changes in …

… ) and kept on ice for 10 min (according to manufacturer’s instructions). The sample was
then sonicated at 4 C with full power for 15 min (30 s off/on cycles) using the BioRuptor plus (…

Upon heterologous overexpression, many proteins misfold or aggregate, thus resulting in
low functional yields. Human acetylcholinesterase (hAChE), an enzyme mediating synaptic …

The calcitic skeletal elements of many organisms contain small amounts of proteins and
glycoproteins which are incorporated within single crystals of calcite. Extraction and partial …

A protein design strategy was developed to specifically enhance the rate of association (k on)
between a pair of proteins without affecting the rate of dissociation (k off). The method is …

… All these observations demonstrate that polymorph specificity is dependent upon the
amino acid sequence, the conformation of specific protein(s) in the mollusk shell and the …

Computational design is a test of our understanding of enzyme catalysis and a means of
engineering novel, tailor-made enzymes. While the de novo computational design of …

Assemblies of glycoproteins from within the mineralized tissues of sea urchins and mollusks
both interact in vitro in a similar manner with growing calcite crystals. A protein‐rich fraction, …