The Wnt family of secreted ligands act through many receptors to stimulate distinct intracellular
signalling pathways in embryonic development, in adults and in disease processes. …

Gli zinc-finger proteins are transcription factors involved in the intracellular signal transduction
controlled by the Hedgehog family of secreted molecules. They are frequently mutated in …

In the last four to five years, the view that G protein–coupled receptors (GPCRs) function as
monomeric proteins has been challenged by numerous studies, which suggests that GPCRs …

The ability to perturb genes in human cells is crucial for elucidating gene function and holds
great potential for finding therapeutic targets for diseases such as cancer. To extend the …

Heptahelical receptors that interact with heterotrimeric G proteins represent the largest family
of proteins involved in signal transduction across biological membranes. Although these …

… Lysates were sonicated (3 3 10 s bursts with 3 s rest in between) on ice at 65% amplitude
using a Qsonica with a CL-18 probe. Lysates and aliquots were centrifuged for 30 min at …

… (C) Recombinant GST-WTX and myelin basic protein (MBP)–AXIN1 synergize to degrade
35 S-labeled β-catenin in Xenopus egg extracts. Graphic representation of 35 S-labeled β-…

… 3b, bacterially produced and purified glutathione S-transferase (GST)-tagged WDR23 (DCAF11)
and H326 (DCAF8) were indeed able to interact with the purified recombinant DDB1 …

The Wnt-β-catenin signaling pathway is an evolutionarily conserved cell-cell communication
system that is important for stem cell renewal, cell proliferation and cell differentiation both …

… Stabilization of protein conformation(s) that are compatible with their export from the ER
using small, cell-permeable ligands may represent a generally applicable rescue strategy. The …