Selectivity of antimicrobial peptides: a complex interplay of multiple equilibria

S Bobone, L Stella - Antimicrobial Peptides: Basics for Clinical Application, 2019 - Springer
… (a) Optical and fluorescence microscopy image of a labeled ubiquicidin analogue (visible
by the green fluorescence) selectively binding to S. aureus bacteria, in co-culture with isolated …

From liposomes to cells: Filling the gap between physicochemical and microbiological studies of the activity and selectivity of host‐defense peptides

F Savini, S Bobone, D Roversi, ML Mangoni… - Peptide …, 2018 - Wiley Online Library
… Filippo Savini and Sara Bobone contributed equally to this study. … Filippo Savini received
a B.Sc. degree in Biology (2011) and a MSc in Molecular and Cellular Biology (2014), from the …

Fluorescence spectroscopy and molecular dynamics simulations in studies on the mechanism of membrane destabilization by antimicrobial peptides

G Bocchinfuso, S Bobone, C Mazzuca… - Cellular and molecular …, 2011 - Springer
… This value is maximum when the probe’s dynamics is restricted and/or slow (compared to
the fluorescence lifetime), and approaches zero when the fluorophore’s motions are fast and …

[HTML][HTML] On the purported “backbone fluorescence” in protein three-dimensional fluorescence spectra

…, SS Korsholm, NHB Bahring, S Bobone, S Tayyab… - Rsc Advances, 2016 - pubs.rsc.org
In this study, several proteins (albumin, lysozyme, insulin) and model compounds (Trp, Tyr,
homopolypeptides) were used to demonstrate the origin of the fluorescence observed upon …

[HTML][HTML] Molecular investigation of SARS–CoV-2 proteins and their interactions with antiviral drugs

P Calligari, S Bobone, G Ricci, A Bocedi - Viruses, 2020 - mdpi.com
… N, O, C and S atoms are colored in blue, red, gray and … N, O, C and S atoms are colored
in blue, red, gray and yellow … N, O, C and S atoms are colored in blue, red, gray and yellow …

[HTML][HTML] Binding of an antimicrobial peptide to bacterial cells: Interaction with different species, strains and cellular components

F Savini, MR Loffredo, C Troiano, S Bobone… - … et Biophysica Acta (BBA …, 2020 - Elsevier
Antimicrobial peptides (AMPs) selectively kill bacteria by disrupting their cell membranes,
and are promising compounds to fight drug-resistant microbes. Biophysical studies on model …

Inoculum effect of antimicrobial peptides

MR Loffredo, F Savini, S Bobone… - Proceedings of the …, 2021 - National Acad Sciences
… coli ATCC 25922 and the Gram-positive S. epidermidis ATCC 12228 were evaluated by …
coli and S. epidermidis were grown in MHB at 37 C to a mid-log phase (6 × 10 8 and 2 × 10 …

A reassessment of synchronous fluorescence in the separation of Trp and Tyr contributions in protein emission and in the determination of conformational changes

S Bobone, M van de Weert, L Stella - Journal of Molecular Structure, 2014 - Elsevier
… The integration time was always 1 s with a step size of 1 nm. All spectra were corrected by …
equal to λ ; however, according to Kasha’s rule the shape of the emission spectrum of a single …

[HTML][HTML] Membrane perturbation by the antimicrobial peptide PMAP-23: a fluorescence and molecular dynamics study

…, JY Kim, A Palleschi, G Grande, S Bobone… - … et Biophysica Acta (BBA …, 2009 - Elsevier
Several bioactive peptides exert their biological function by interacting with cellular membranes.
Structural data on their location inside lipid bilayers are thus essential for a detailed …

Phosphatidylserine lateral organization influences the interaction of influenza virus matrix protein 1 with lipid membranes

S Bobone, M Hilsch, J Storm, V Dunsing… - Journal of …, 2017 - Am Soc Microbiol
… Image time-stacks of 100 scans were collected at time intervals of 1.155 s per frame (4.325
ms per line). For each sample, a total of six measurements at different positions were …