Recent structural and biophysical results have provided unprecedented insights into the
structure and function of the bacterial RNA polymerase holoenzyme as it goes through the steps …

Transcription initiation is the crucial focal point of gene expression in prokaryotes. The key
players in this process, sigma factors (σs), associate with the catalytic core RNA polymerase …

Recently determined structures of the Escherichia coli catabolite activator protein (CAP) in
complex with DNA, and in complex with the RNA polymerase α subunit C-terminal domain (…

Rifampicin (Rif) is one of the most potent and broad spectrum antibiotics against bacterial
pathogens and is a key component of anti-tuberculosis therapy, stemming from its inhibition of …

The X-ray crystal structure of Thermus aquaticus core RNA polymerase reveals a "crab claw"–shaped
molecule with a 27 Å wide internal channel. Located on the back wall of the …

The crystal structure of Thermus aquaticus RNA polymerase holoenzyme (α 2 ββ′ωσ A )
complexed with a fork-junction promoter DNA fragment has been determined by fitting high-…

The crystal structure of the initiating form of Thermus aquaticus RNA polymerase, containing
core RNA polymerase (α 2 ββ′ω) and the promoter specificity σ subunit, has been …

… Our measured kcat ($15 s … , SARS-CoV-2 nsp13-Val570 is Ile in SARSCoV nsp13) but
not to kcat values for SARS-CoV His6-nsp13 and glutathione S-transferase (GST)-nsp13 (0.2 s …

… Based on the structure, we have re’s; see supplemental data … makes van der Waal’s contact
with the edge of the –32 T ring… Several side chains make van der Waal’s interactions with the …

… The elongation rate for the prokaryotic RNAPs is 50 to 100 nt/s, while the eukaryotic enzymes
are even slower, at 5 to 20 nt/s. This is contrasted with 300 to 1000 nt/s elongation rates for …