… DNA polymerase (Finnzymes) and the following program: 98 C for 30 s followed by 35 cycles
of 98 C for 10 s, 62 C for 20 s and 72 C for 10 s, with a final extension at 72 C for 2 min. The …

The discovery of interchromosomal interactions in higher eukaryotes points to a functional
interplay between genome architecture and gene expression, challenging the view of …

… Neonatal mouse liver cells were dispersed by immediate mashing through a 70-mm nylon
cell strainer into Dulbecco’s modified Eagle’s medium. The 3C assays were done essentially …

… each 35 S-labeled protein was mixed in 300 μl of BB-A with the GST beads taken at volumes
giving equal amounts of each immobilized protein as measured by 35 S … of the 35 S signal. …

… -bound loci function as barrier insulators in the distantly related yeast, S. cerevisiae and …
We developed a barrier assay in S. pombe that is similar to the barrier assay in S. cerevisiae…

The genome is spatially organized inside nuclei, with chromosomes and genes occupying
preferential positions relative to each other and to various nuclear landmarks. What drives this …

Most of the transcription factors, RNA polymerases and enhancer binding factors are absent
from condensed mitotic chromosomes. In contrast, epigenetic marks of active and inactive …

Chromosome conformation capture (3C) is a powerful technique for analyzing spatial chromatin
organization in vivo. Technical variants of the assay ('4C') allow the systematic detection …

TAL1 is an important regulator of hematopoiesis and its expression is tightly controlled
despite complexities in its genomic organization. It is frequently misregulated in T-cell acute …

… 30 s, 60C for 30 s, and 72C for 30 s. The expression levels of β- actin did not change under
the different experimental conditions and were used as an internal control to normalize each …