RNA N6-methyladenosine and the regulation of RNA localization and function in the brain

SU Madugalle, K Meyer, DO Wang, TW Bredy - Trends in neurosciences, 2020 - cell.com
… Investigations into the roles of m 6 A in RNA biology are rapidly expanding but require
new techniques to fully understand m 6 A’s role in RNA localization in the brain. …

[HTML][HTML] Fear extinction is regulated by the activity of long noncoding RNAs at the synapse

…, A Periyakaruppiah, SU Madugalle… - Nature …, 2023 - nature.com
… 120 s and then extinction trained with 60 non-reinforced 120 s CS presentations with a 5 s
… B (24 h later) and presented with a 3 120 s CS with 120 s ITI. Memory was calculated as the …

[PDF][PDF] ADRAM is an experience-dependent long noncoding RNA that drives fear extinction through a direct interaction with the chaperone protein 14-3-3

…, EL Zajaczkowski, LJ Leighton, SU Madugalle… - Cell reports, 2022 - cell.com
Here, we used RNA capture-seq to identify a large population of lncRNAs that are expressed
in the infralimbic prefrontal cortex of adult male mice in response to fear-related learning. …

The DNA modification N6-methyl-2'-deoxyadenosine (m6dA) drives activity-induced gene expression and is required for fear extinction

…, J Yin, SU Madugalle, Z Wang, S Nainar… - Nature …, 2019 - nature.com
… 60 non-reinforced 120 s conditioned stimulus presentations (at 5-s intervals). For behavior …
assessed during three 120 s conditioned stimulus presentations (with 120 s intertribal interval)…

Synapse-enriched m6A-modified Malat1 interacts with the novel m6A reader, DPYSL2, and is required for fear-extinction memory

SU Madugalle, WS Liau, Q Zhao, X Li… - Journal of …, 2023 - Soc Neuroscience
s pre-fear-conditioning habituation period, which was followed by three pairings of a 120 s,
80 dB white noise conditioned stimulus (CS) with a 1 s… nonreinforced 120 s CS presentations (…

The DNA repair-associated protein Gadd45γ regulates the temporal coding of immediate early gene expression within the prelimbic prefrontal cortex and is required …

…, EL Zajaczkowski, Z Wang, SU Madugalle… - Journal of …, 2019 - Soc Neuroscience
s, 80 dB, pure tone stimulus (CS) with a 1 s, 0.7 mA, foot shock (US), separated by an
empty 10 s … The pretrial interval, intertrial interval, and post-trial interval were all 120 s long. …

Dynamic regulation of Z-DNA in the mouse prefrontal cortex by the RNA-editing enzyme Adar1 is required for fear extinction

…, EL Zajaczkowski, LJ Leighton, SU Madugalle… - Nature …, 2020 - nature.com
DNA forms conformational states beyond the right-handed double helix; however, the
functional relevance of these noncanonical structures in the brain remains unknown. Here we …

Localised Cdr1as activity is required for fear extinction memory

…, Q Zhao, WS Liau, H Gong, SU Madugalle… - … of Learning and …, 2023 - Elsevier
… , pure 16 kHz tone (CS) co-terminating with a 1 s 0.7 mA foot shock (US) in CTX A. Animals
… but the ITI between CS presentations was 5 s. The percentage of time the animal remained …

Fear extinction is regulated by long noncoding RNA activity at the synapse

…, PR Marshall, A Periyakaruppiah, SU Madugalle… - BioRxiv, 2022 - biorxiv.org
… HEK293T cells were maintained in a medium containing Dulbecco’s modified eagle
medium (DMEM) and high glucose (Gibco 11965) with 5% FBS and 1% penicillin-streptomycin. …

DNA G-quadruplex is a transcriptional control device that regulates memory

…, LJ Leighton, SU Madugalle… - Journal of …, 2024 - Soc Neuroscience
The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA.
Here we report that DNA G-quadruplex (G4-DNA) accumulates in neurons, in an experience-…