… We used the non-fluorescent substrate (S) dihydrorhodamine 6G which after oxidation yields
the … We have recorded the autocorrelation functions of 10 s and 300 s traces from single …

… NT sequence, was subjected to point mutagenesis using the QuickChange site-directed
mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s …

… cDNA was denatured for 30 s at 95C and then subjected to 40 cycles of 95C for 15 s and
60C for 30 s. The primers were obtained from Sigma (KiCqStart® primers): TGFB1, SMAD2, …

… Denaturation was at 96C for 30 s, annealing was at 65C for 60 s, and elongation was at
72C for 120 s. Twenty-five cycles were run. (GenAmp PCR System 2400, Perkin–Elmor). …

… frame of 85–101 s (including 15 s pre-bleach measurements). … τ/s gives time shift per s. j
The scatter plot summarizes FCCS … Data are based on two to eight 10 s acquisition periods per …

… for 30 s; thereafter, only 488 nm excitation was used for 30 s. Already during the first 30 s, the
… During the second 30 s, as the 594 nm excitation was switched off, the baseline in the red …

… After 1 h, the dispersion was sonicated using a tip sonicator (CV18 model, Chemical Instruments
AB) set at 40% amplitude in pulse mode (10 s on followed by 10 s off) for 15 min of total …

… The sedimentation velocity (v s ) of spherical particles with a diameter a is given by:(9) v s
= g ρ p − ρ a 2 18 η where g is the gravitational acceleration, ρ p and ρ are the densities of …

… magna’s alimentary canal tissues without any toxicity effect leading to the death of the …
400 μm 2 /s in in water and thereby a diffusion coefficient of about 200 μm 2 /s in DMSO. This …

… In the following, we use the index S to indicate coordinates … located at an arbitrary z S position
is R z = R 0 + z S tan α. Here, R … For this, let d S = ( x S 2 + y S 2 ) 1 / 2 denote the distance …