Super-resolution imaging methods now can provide spatial resolution that is well below the
diffraction limit approaching virtually molecular resolution. They can be applied to biological …

Molecular optical photoswitches based on fluorescent proteins and organic dyes are
fundamental for super-resolution fluorescence imaging and tracking methods. Precise control of …

… Laine RF, Kaminski Schierle GS, van de Linde S, Kaminski CF., Methods Appl Fluoresc 4(2), …
Proppert S, Wolter S, Holm T, Klein T, van de Linde S, Sauer M., Opt Express 22(9), 2014 …

Direct stochastic optical reconstruction microscopy (dSTORM) uses conventional
fluorescent probes such as labeled antibodies or chemical tags for subdiffraction resolution …

Super-resolution microscopy can unravel previously hidden details of cellular structures but
requires high irradiation intensities to use the limited photon budget efficiently. Such high …

… Heilemann M, van de Linde S, Mukherjee A, Sauer M. Super-Resolution Imaging with Small
Organic Fluorophores. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. … Laine RF …

To the Editor: In recent years, there has been a dramatic increase in the use of localization
microscopy: that is, superresolution fluorescence imaging by stochastic photoswitching, …

Super-resolution fluorescence imaging can provide insights into cellular structure and
organization with a spatial resolution approaching virtually electron microscopy. Among all the …

The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the
difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical …

… dSTORM was performed as described elsewhere (van de Linde et al., 2011), using an
inverted microscope (Olympus IX-71) equipped with an oil-immersion objective (×60, NA 1.45; …