We present a proof-of-concept methodology for efficiently optimizing a chemical trait by
using an artificial evolutionary workflow. We demonstrate this by optimizing the efficacy of …

Multi-drug strategies have been attempted to prolong the efficacy of existing antibiotics, but
with limited success. Here we show that the evolution of multi-drug-resistant Escherichia coli …

The reconstructed in vitro translation system known as the PURE system has been used in
a variety of cell‐free experiments such as the expression of native and de novo proteins as …

Attempts to create complex molecular systems that mimic parts of cellular systems using a
bottom-up approach have become important in the field of biology. Among various molecular …

Cell-free protein expression systems have been widely used for synthetic biology and metabolic
engineering applications in recent years. Yet little is known about protein expression in …

Synthetic biology has become a promising field that aims at developing and using tools to
work with recombinant DNA. In this field, cell-free protein expression systems have become a …

The number of effective antibiotics is rapidly decreasing due to the emergence of resistant
bacteria, which necessitates the development of not only new, but novel strategies for …

The recombinant in vitro translation system known as the PURE system has been used in a
variety of cell-free experiments such as expression of native and de novo proteins as well as …

We present a proof of concept methodology to efficiently optimize a chemical trait using an
artificial evolutionary workflow. We demonstrate this by optimizing the efficacy of antimicrobial …

The use of chemotherapeutics agents in the treatment of bacterial infections have resulted
in the emergence of multidrug resistant pathogens. Clinically, with single and even multiple …