In skeletal muscle, nebulin stabilizes and regulates the length of thin filaments, but the
underlying mechanism remains nebulous. In this work, we used cryo–electron tomography and …

The function of actin is coupled to the nucleotide bound to its active site. ATP hydrolysis is
activated during polymerization; a delay between hydrolysis and inorganic phosphate (P i ) …

The dynamic turnover of actin filaments (F-actin) controls cellular motility in eukaryotes and
is coupled to changes in the F-actin nucleotide state 1 , 2 – 3 . It remains unclear how F-actin …

Actin undergoes structural transitions during polymerization, ATP hydrolysis, and subsequent
release of inorganic phosphate. Several actin-binding proteins sense specific states during …

Single particle cryo-EM requires full automation to allow high-throughput structure determination.
Although software packages exist where parts of the cryo-EM pipeline are automated, …

Actin filaments (F-actin) are a key component of eukaryotic cells. Whether serving as a scaffold
for myosin or using their polymerization to push onto cellular components, their function is …

During their life cycle, apicomplexan parasites, such as the malaria parasite Plasmodium
falciparum, use actomyosin-driven gliding motility to move and invade host cells. For this …

The molecular motor myosin undergoes a series of major structural transitions during its
force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and …

Structure determination of filamentous molecular complexes involves the selection of filaments
from cryo-EM micrographs. The automatic selection of helical specimens is particularly …

The leading cause of dementia in adults is Alzheimer's disease (AD), which accounts for more
than 80% of dementia cases worldwide (1). This progressive neurodegenerative disorder …