Cellular processes often depend on stable physical associations between proteins. Despite
recent progress, knowledge of the composition of human protein complexes remains limited. …

Macromolecular complexes are essential to conserved biological processes, but their
prevalence across animals is unclear. By combining extensive biochemical fractionation with …

One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally
unannotated (orphans). Many map to distant clades such as Archaea, suggesting involvement …

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) and the associated
proteins (Cas) comprise a system of adaptive immunity against viruses and plasmids in …

Physical and functional interactions define the molecular organization of the cell. Genetic
interactions, or epistasis, tend to occur between gene products involved in parallel pathways or …

Macromolecular assemblies involving membrane proteins (MPs) serve vital biological roles
and are prime drug targets in a variety of diseases 1 . Large-scale affinity purification studies …

Efforts to map the Escherichia coli interactome have identified several hundred macromolecular
complexes, but direct binary protein-protein interactions (PPIs) have not been surveyed …

Bacterial cell envelope protein (CEP) complexes mediate a range of processes, including
membrane assembly, antibiotic resistance and metabolic coordination. However, only limited …

Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous
macromolecular networks on a proteome scale, but current methods are experimentally laborious, …

As the interface between a microbe and its environment, the bacterial cell envelope has
broad biological and clinical significance. While numerous biosynthesis genes and pathways …