We describe an engineered family of highly antigenic molecules based on GFP-like
fluorescent proteins. These molecules contain numerous copies of peptide epitopes and …

The neocortex contains a multitude of cell types that are segregated into layers and functionally
distinct areas. To investigate the diversity of cell types across the mouse neocortex, here …

The thalamus receives input from 3 distinct cortical layers, but input from only 2 of these has
been well characterized. We therefore investigated whether the third input, derived from …

In mammals, subplate neurons (SPNs) are among the first generated cortical neurons. While
most SPNs exist only transiently during development, a number of SPNs persist among …

Efficient retrograde access to projection neurons for the delivery of sensors and effectors
constitutes an important and enabling capability for neural circuit dissection. Such an approach …

Activity in the motor cortex predicts movements, seconds before they are initiated. This
preparatory activity has been observed across cortical layers, including in descending pyramidal …

Although messenger RNA (mRNA) translation is a fundamental biological process, it has
never been imaged in real time in vivo with single-molecule precision. To achieve this, we …

Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples.
However, they lose their fluorescence after heavy fixation, hindering applications such as …

Subplate neurons (SPNs) are a population of neurons in the mammalian cerebral cortex that
exist predominantly in the prenatal and early postnatal period. Loss of SPNs prevents the …

ClpS is an adaptor protein that interacts with ClpA and promotes degradation of proteins
with N-end rule degradation motifs (N-degrons) by ClpAP while blocking degradation of …