We have designed a tagged probe [sphingolipid binding domain (SBD)] to facilitate the
tracking of intracellular movements of sphingolipids in living neuronal cells. SBD is a small …

During the Drosophila life‐cycle two sets of neuromuscular junctions are generated: the
embryonic/larval NMJs develop during the first half, followed by the period of metamorphosis …

Intracellular accumulation of lipids and swollen dysfunctional lysosomes are linked to
several neurodegenerative diseases, including lysosomal storage disorders (LSD). Detailed …

Absolute (molar) quantification of proteins determines their molar ratios in complexes,
networks, and metabolic pathways. MS Western workflow is employed to determine molar …

We have used laser capture microdissection (LCM) and fluorescence microscopy to isolate
genetically labeled neurons from the Drosophila melanogaster brain. From native thin …

Sphingolipid metabolites are involved in the regulation of autophagy, a degradative
recycling process that is required to prevent neuronal degeneration. Drosophila blue cheese …

During its life cycle, Drosophila makes two sets of neuromuscular junctions (NMJs),
embryonic/larval and adult, which serve distinct stage‐specific functions. During metamorphosis, the …

Saposin deficiency is a childhood neurodegenerative lysosomal storage disorder (LSD) that
can cause premature death within three months of life. Saposins are activator proteins that …

Apical domains of epithelial cells often undergo dramatic changes during morphogenesis to
form specialized structures, such as microvilli. Here, we addressed the role of lipids during …

Background The uptake and intracellular trafficking of sphingolipids, which self-associate
into plasma membrane microdomains, is associated with many pathological conditions, …