[HTML][HTML] Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
We present two standards developed by the Genomic Standards Consortium (GSC) for
reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum …
reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum …
[HTML][HTML] Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP)
S Tighe, E Afshinnekoo, TM Rock… - Journal of …, 2017 - ncbi.nlm.nih.gov
The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular
Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on …
Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on …
[PDF][PDF] A global metagenomic map of urban microbiomes and antimicrobial resistance
…, L Shi, T Shi, R Siam, H Suzuki, SW Tighe… - Cell, 2021 - cell.com
We present a global atlas of 4,728 metagenomic samples from mass-transit systems in 60
cities over 3 years, representing the first systematic, worldwide catalog of the urban microbial …
cities over 3 years, representing the first systematic, worldwide catalog of the urban microbial …
[HTML][HTML] Comprehensive benchmarking and ensemble approaches for metagenomic classifiers
Background One of the main challenges in metagenomics is the identification of microorganisms
in clinical and environmental samples. While an extensive and heterogeneous set of …
in clinical and environmental samples. While an extensive and heterogeneous set of …
[HTML][HTML] Real-time DNA sequencing in the Antarctic dry valleys using the Oxford Nanopore sequencer
…, E Zaikova, DS Goerlitz, Y Bai, SW Tighe - Journal of …, 2017 - ncbi.nlm.nih.gov
The ability to sequence DNA outside of the laboratory setting has enabled novel research
questions to be addressed in the field in diverse areas, ranging from environmental …
questions to be addressed in the field in diverse areas, ranging from environmental …
[PDF][PDF] Geospatial resolution of human and bacterial diversity with city-scale metagenomics
The panoply of microorganisms and other species present in our environment influence human
health and disease, especially in cities, but have not been profiled with metagenomics at …
health and disease, especially in cities, but have not been profiled with metagenomics at …
Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study
High-throughput RNA sequencing (RNA-seq) greatly expands the potential for genomics
discoveries, but the wide variety of platforms, protocols and performance capabilitites has …
discoveries, but the wide variety of platforms, protocols and performance capabilitites has …
Molecular signatures suggest a major role for stromal cells in development of invasive breast cancer
T Casey, J Bond, S Tighe, T Hunter, L Lintault… - Breast cancer research …, 2009 - Springer
Background Breast cancer invasion and metastasis involves both epithelial and stromal
changes. Our objective was to delineate the pivotal role stroma plays in invasion by comparing …
changes. Our objective was to delineate the pivotal role stroma plays in invasion by comparing …
Performance assessment of DNA sequencing platforms in the ABRF Next-Generation Sequencing Study
Assessing the reproducibility, accuracy and utility of massively parallel DNA sequencing
platforms remains an ongoing challenge. Here the Association of Biomolecular Resource …
platforms remains an ongoing challenge. Here the Association of Biomolecular Resource …
[HTML][HTML] Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step
EA Bruce, ML Huang, GA Perchetti, S Tighe… - PLoS …, 2020 - journals.plos.org
The ongoing COVID-19 pandemic has created an unprecedented need for rapid diagnostic
testing. The World Health Organization (WHO) recommends a standard assay that includes …
testing. The World Health Organization (WHO) recommends a standard assay that includes …