Highlights • CRISPR/Cas9 is the method of choice for site-directed genetic or transcriptional
manipulation. • Genomic loci differ in chromatin configuration which affects Cas9 functioning…

CRISPR/Cas9-based homing gene drives have emerged as a potential new approach to
mosquito control. While attempts have been made to develop such systems in Aedes aegypti, …

Making discrete and precise genetic changes to wild populations has been proposed as a
means of addressing some of the world's most pressing ecological and public health …

CRISPR/Cas gene drives can bias transgene inheritance through different mechanisms.
Homing drives are designed to replace a wild-type allele with a copy of a drive element on the …

The increasing prevalence of insecticide resistance and the ongoing global burden of vector-borne
diseases have encouraged new efforts in mosquito control. For Aedes aegypti, the …

CRISPR–Cas9-based “gene drive” technologies have been proposed as a novel and effective
means of controlling human diseases vectored by mosquitoes. However, more complex …

The introgression of genetic traits through gene drive may serve as a powerful and widely
applicable method of biological control. However, for many applications, a self-perpetuating …

Aedes aegypti, the yellow fever mosquito, is the main vector of several major pathogens
including yellow fever, dengue, Zika and chikungunya viruses. Classical mosquito control …

Aedes aegypti is the main vector of several major pathogens including dengue, Zika and
chikungunya viruses. Classical mosquito control strategies utilizing insecticides are threatened …

Aedes aegypti and Aedes albopictus mosquitoes are vectors of the RNA viruses
chikungunya (CHIKV) and dengue that currently have no specific therapeutic treatments. The …