Allelic exclusion underpins antigenic variation and immune evasion in African trypanosomes.
These bloodstream parasites use RNA polymerase-I (pol-I) to transcribe just one telomeric …

Highly selective gene expression is a key requirement for antigenic variation in several
pathogens, allowing evasion of host immune responses and maintenance of persistent infections …

The largest gene families in eukaryotes are subject to allelic exclusion, but mechanisms
underpinning single allele selection and inheritance remain unclear. Here, we describe a …

CRISPR/Cas9 technology has been developing rapidly in the field of parasitology, allowing
for the dissection of molecular processes with unprecedented efficiency. Optimization and …

Chemotherapy continues to have a major impact on reducing the burden of disease caused
by trypanosomatids. Unfortunately though, the mode-of-action (MoA) of antitrypanosomal …

African trypanosomes are lethal human and animal parasites that use antigenic variation for
evasion of host adaptive immunity. To facilitate antigenic variation, trypanosomes dedicate …

The long and complex Trypanosoma brucei development in the tsetse fly vector culminates
when parasites gain mammalian infectivity in the salivary glands. A key step in this process is …

Primary defects in motile cilia result in dysfunction of the apparatus responsible for generating
fluid flows. Defects in these mechanisms underlie disorders characterized by poor mucus …

The ability to simultaneously assess every gene in a genome for a role in a particular process
has obvious appeal. This protocol describes how to perform genome-scale RNAi library …

Kinetoplastid parasites cause lethal diseases in humans and animals. The kinetoplast itself
contains the mitochondrial genome, comprising a huge, complex DNA network that is also …