By recording the full fluorescence spectra and super-resolved positions of ∼10 6 individual
polarity-sensing solvatochromic molecules, we reveal compositional heterogeneity in the …

The application of electron microscopy to hydrated biological samples has been limited by
high-vacuum operating conditions. Traditional methods utilize harsh and laborious sample …

Visualization of the nanoscale organization of cell membranes remains challenging because
of the lack of appropriate fluorescent probes. Herein, we introduce a new design concept …

Single-molecule localization microscopy (SMLM), while well established for cultured cells,
is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-…

Conspectus As an elegant integration of the spatial and temporal dimensions of single-molecule
fluorescence, single-molecule localization microscopy (SMLM) overcomes the …

Neural circuit function depends on the pattern of synaptic connections between neurons
and the strength of those connections. Synaptic strength is determined by both postsynaptic …

Recent years have witnessed the development of single-molecule localization microscopy
as a generic tool for sampling diverse biologically relevant information at the super-resolution …

The discovery and rise of graphene were historically enabled by its ∼10% optical contrast
on specialized substrates like oxide-capped silicon. However, substantially lower contrast is …

Under ambient conditions, the behavior of a solid surface is often dominated by a molecularly
thin adsorbed layer (adlayer) of small molecules. Here we develop an optical approach to …

The incorporation of exogenous molecules into live cells is essential for both biological
research and therapeutic applications. In particular, for the emerging field of super-resolution …