Efficient Correction of Oncogenic KRAS and TP53 Mutations through CRISPR Base Editing
S Sayed, OA Sidorova, A Hennig, M Augsburg… - Cancer research, 2022 - AACR
Repairing KRAS mutations with base editors can be used for providing a better understanding
of RAS biology and may lay the foundation for improved treatments for KRAS -mutant …
of RAS biology and may lay the foundation for improved treatments for KRAS -mutant …
[HTML][HTML] Deletion of lrrk2 causes early developmental abnormalities and age-dependent increase of monoamine catabolism in the zebrafish brain
LRRK2 gain-of-function is considered a major cause of Parkinson’s disease (PD) in humans.
However, pathogenicity of LRRK2 loss-of-function in animal models is controversial. Here …
However, pathogenicity of LRRK2 loss-of-function in animal models is controversial. Here …
[HTML][HTML] High-resolution fabrication of nanopatterns by multistep iterative miniaturization of hot-embossed prestressed polymer films and constrained shrinking
S Sayed, PR Selvaganapathy - Microsystems & Nanoengineering, 2022 - nature.com
The fabrication of nanostructures and nanopatterns is of crucial importance in microelectronics,
nanofluidics, and the manufacture of biomedical devices and biosensors. However, the …
nanofluidics, and the manufacture of biomedical devices and biosensors. However, the …
[HTML][HTML] CRISPR/Cas9 as a tool to dissect cancer mutations
S Sayed, M Paszkowski-Rogacz, LT Schmitt… - Methods, 2019 - Elsevier
The CRISPR/Cas9 system is transforming many biomedical disciplines, including cancer
research. Through its flexible programmability and efficiency to induce DNA double strand …
research. Through its flexible programmability and efficiency to induce DNA double strand …
[HTML][HTML] Quantification of evolved DNA-editing enzymes at scale with DEQSeq
…, J Posorski, F Lansing, M Jelicic, M Jain, S Sayed… - Genome Biology, 2023 - Springer
We introduce DEQSeq, a nanopore sequencing approach that rationalizes the selection of
favorable genome editing enzymes from directed molecular evolution experiments. With the …
favorable genome editing enzymes from directed molecular evolution experiments. With the …
Using CRISPR-Cas9 to Dissect Cancer Mutations in Cell Lines
S Sayed, D Sürün, J Mircetic, OA Sidorova… - Cancer Cell Biology …, 2022 - Springer
The CRISPR-Cas9 technology has revolutionized the scope and pace of biomedical research,
enabling the targeting of specific genomic sequences for a wide spectrum of applications. …
enabling the targeting of specific genomic sequences for a wide spectrum of applications. …
[HTML][HTML] RNAi-Mediated Screen of Primary AML Cells Nominates MDM4 as a Therapeutic Target in NK-AML with DNMT3A Mutations
OA Sidorova, S Sayed, M Paszkowski-Rogacz… - Cells, 2022 - mdpi.com
DNA-methyltransferase 3A (DNMT3A) mutations belong to the most frequent genetic
aberrations found in adult acute myeloid leukemia (AML). Recent evidence suggests that these …
aberrations found in adult acute myeloid leukemia (AML). Recent evidence suggests that these …
[HTML][HTML] Antimicrobial potentials of natural products against multidrug resistance pathogens: A comprehensive review
AH Elmaidomy, NH Shady, KM Abdeljawad… - RSC …, 2022 - pubs.rsc.org
Antibiotic resistance is one of the critical issues, describing a significant social health complication
globally. Hence, the discovery of novel antibiotics has acquired an increased attention …
globally. Hence, the discovery of novel antibiotics has acquired an increased attention …
[HTML][HTML] Sterols and triterpenes: Antiviral potential supported by in-silico analysis
The acute respiratory syndrome caused by the novel coronavirus (SARS-CoV-2) caused
severe panic all over the world. The coronavirus (COVID-19) outbreak has already brought …
severe panic all over the world. The coronavirus (COVID-19) outbreak has already brought …
Artificial neural networks applications in partially shaded PV systems
… (ANNs) used extensively in analysing partially shaded PV systems. This work reviews the
applications of ANNs in various aspects of partially shaded PV systems. The application of …
applications of ANNs in various aspects of partially shaded PV systems. The application of …