CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.

SS Sugano, M Shirakawa, J Takagi… - Plant and Cell …, 2014 - academic.oup.com
Targeted genome modification technologies are key tools for functional genomics. The
clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease …

Stomagen positively regulates stomatal density in Arabidopsis

SS Sugano, T Shimada, Y Imai, K Okawa, A Tamai… - Nature, 2010 - nature.com
Stomata in the epidermal tissues of leaves are valves through which passes CO 2 , and as
such they influence the global carbon cycle 1 . The two-dimensional pattern and density of …

[HTML][HTML] Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants

Y Osakabe, T Watanabe, SS Sugano, R Ueta… - Scientific reports, 2016 - nature.com
Genome editing using the CRISPR/Cas9 system can be used to modify plant genomes,
however, improvements in specificity and applicability are still needed in order for the editing …

Enhancement of leaf photosynthetic capacity through increased stomatal density in Arabidopsis

Y Tanaka, SS Sugano, T Shimada… - New …, 2013 - Wiley Online Library
… g s between WT and ST-RNAi were not significant under any light conditions. Differences in
g s between … s −1 and above, although the upregulation of g s in ST-OX was observed to be …

[HTML][HTML] Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9

R Ueta, C Abe, T Watanabe, SS Sugano, R Ishihara… - Scientific reports, 2017 - nature.com
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for
various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding …

Two Y-chromosome-encoded genes determine sex in kiwifruit

…, E Varkonyi-Gasic, IM Henry, SS Sugano… - Nature plants, 2019 - nature.com
Dioecy, the presence of male and female individuals, has evolved independently in multiple
flowering plant lineages 1 , 2 – 3 . Although theoretical models for the evolution of dioecy, …

[HTML][HTML] Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha

SS Sugano, R Nishihama, M Shirakawa, J Takagi… - PloS one, 2018 - journals.plos.org
Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based
genome editing has already been demonstrated for this plant, the efficiency …

[HTML][HTML] Higher stomatal density improves photosynthetic induction and biomass production in Arabidopsis under fluctuating light

K Sakoda, W Yamori, T Shimada, SS Sugano… - Frontiers in Plant …, 2020 - frontiersin.org
s under initial dark condition. In addition, ST-OX and epf1 showed initially faster g s induction
and, at the later phase, slower g s … of A and g s , we calculated A induction and g s induction …

[HTML][HTML] Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

SS Sugano, H Suzuki, E Shimokita, H Chiba, S Noji… - Scientific reports, 2017 - nature.com
Mushroom-forming basidiomycetes produce a wide range of metabolites and have great
value not only as food but also as an important global natural resource. Here, we demonstrate …

Positive and negative peptide signals control stomatal density

T Shimada, SS Sugano, I Hara-Nishimura - Cellular and Molecular Life …, 2011 - Springer
The stoma is a micro valve found on aerial plant organs that promotes gas exchange between
the atmosphere and the plant body. Each stoma is formed by a strict cell lineage during …