Cryo-electron tomography is a powerful technique that can faithfully image the native
cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo-…

Macrophages are central orchestrators of the tissue response to injury, with distinct macrophage
activation states playing key roles in fibrosis progression and resolution. Identifying key …

The ability to image RNA identity and location with nanoscale precision in intact tissues is of
great interest for defining cell types and states in normal and pathological biological settings…

INTRODUCTION Neural circuits across the brain are composed of structures spanning seven
orders of magnitude in size that are assembled from thousands of distinct protein types. …

We recently developed a method called expansion microscopy, in which preserved
biological specimens are physically magnified by embedding them in a densely crosslinked …

The 26S proteasome is a key player in eukaryotic protein quality control and in the regulation
of numerous cellular processes. Here, we describe quantitative in situ structural studies of …

We developed a new way to engineer complex proteins toward multidimensional
specifications using a simple, yet scalable, directed evolution strategy. By robotically picking …

Expansion microscopy (ExM) is a recently developed technique that enables nanoscale‐resolution
imaging of preserved cells and tissues on conventional diffraction‐limited …

Electron tomography enables three-dimensional (3D) visualization and analysis of the
subcellular architecture at a resolution of a few nanometers. Segmentation of structural …

Lithographic nanofabrication is often limited to successive fabrication of two-dimensional (2D)
layers. We present a strategy for the direct assembly of 3D nanomaterials consisting of …