The three-dimensional positions of atoms in protein molecules define their structure and their
roles in biological processes. The more precisely atomic coordinates are determined, the …

To the Editor: In the field of single-particle analysis of electron cryomicroscopy (cryo-EM) data,
a growing concern that some resolution claims might not be substantiated by the data has …

The ordered assembly of tau protein into abnormal filamentous inclusions underlies many
human neurodegenerative diseases 1 . Tau assemblies seem to spread through specific …

For many years, structure determination of biological macromolecules by cryo-electron
microscopy (cryo-EM) was limited to large complexes or low-resolution models. With recent …

This chapter describes algorithmic advances in the RELION software, and how these are
used in high-resolution cryo-electron microscopy (cryo-EM) structure determination. Since the …

… how images from an electron microscope can be used to study the structures of proteins and
how rapid … We also highlight how the unique characteristics of this technique have already …

RELION, for REgularized LIkelihood OptimizatioN, is an open-source computer program for
the refinement of macromolecular structures by single-particle analysis of electron cryo-…

10.7554/eLife.42166.001 Here, we describe the third major release of RELION. CPU-based
vector acceleration has been added in addition to GPU support, which provides flexibility in …

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virions are surrounded by
a lipid bilayer from which spike (S) protein trimers protrude 1 . Heavily glycosylated S trimers …

Alzheimer’s disease is the most common neurodegenerative disease, and there are no
mechanism-based therapies. The disease is defined by the presence of abundant neurofibrillary …