CRISPR/Cas9 genome engineering strategies allow the directed modification of the
Caenorhabditis elegans genome to introduce point mutations, generate knock-out mutants, and …

Transgenes are prone to progressive silencing due to their structure, copy number, and
genomic location. In C. elegans, repressive mechanisms are particularly strong in the germline …

Efficient and reproducible transgenesis facilitates and accelerates research using genetic
model organisms. Here, we describe a modular safe-harbor transgene insertion (MosTI) for …

Genetic assimilation—the evolutionary process by which an environmentally induced
phenotype is made constitutive—represents a fundamental concept in evolutionary biology. …

Mutations that modulate the activity of ion channels are essential tools to understand the
biophysical determinants that control their gating. Here, we reveal the conserved role played by …

Figure 1. The histamine-gated chloride channel HisCl1 effectively paralyzes transgenic array
animals: AD. Doseresponse relationship and time-course for the paralysis of transgenic …

Figure 1. Using a pan-muscular promoter for single-copy transgene selection: AC.
Characterization of the fluorescence expression pattern from MosSCI insertions of transgenes with …

Neurobeachin (NBEA) was initially identified as a candidate gene for autism. Recently, variants
in NBEA have been associated with neurodevelopmental delay and childhood epilepsy. …

A core tenet of synthetic biology is that well-characterized regulatory elements are essential
for engineering biological systems. Here, we characterize the specificity and expression …

Transgenesis in model organisms is an essential tool for determining the function of protein-coding
genes and non-coding regulatory regions. In Caenorhabditis elegans, injected DNA …