Highlights • Approaches to the structure determination and modelling of macromolecular
assemblies depend on the resolution of their cryo-EM map. • Insights into structure dynamics …

INTRODUCTION Nearly all mycobacterial species are free-living environmental saprophytes.
A few, such as Mycobacterium tuberculosis, have evolved to cause transmissible human …

Interest in applications of protein crystallography to medicine was evident, as the first high-resolution
structures emerged in the 50s and 60s. In Cambridge, Max Perutz and John …

Heterozygous mutations in KMT2B are associated with an early-onset, progressive and
often complex dystonia (DYT28). Key characteristics of typical disease include focal motor …

Of the more than 190 distinct species of Mycobacterium genus, many are economically and
clinically important pathogens of humans or animals. Among those mycobacteria that infect …

Background Krishna Tulsi, a member of Lamiaceae family, is a herb well known for its spiritual,
religious and medicinal importance in India. The common name of this plant is ‘Tulsi’ (or ‘…

Objective To characterize the phenotypic spectrum, molecular genetic findings, and
functional consequences of pathogenic variants in early-onset KCNT1 epilepsy. Methods We …

As the resolutions of Three Dimensional Electron Microscopic reconstructions of biological
macromolecules are being improved, there is a need for better fitting and refinement methods …

The medical and scientific response to emerging and established pathogens is often severely
hampered by ignorance of the genetic determinants of virulence, drug resistance and …

Molecular structures are often fitted into cryo-EM maps by flexible fitting. When this requires
large conformational changes, identifying rigid bodies can help optimize the model-map fit. …