Golden Gate cloning is a prominent DNA assembly tool in synthetic biology for the assembly
of plasmid constructs often used in combinatorial pathway optimization, with a number of …

Prokaryotic cell-free coupled transcription–translation (TX-TL) systems are emerging as a
powerful tool to examine natural product biosynthetic pathways in a test tube. The key …

The polyhydroxyalkanoates (PHAs) are microbially-produced biopolymers that could potentially
be used as sustainable alternatives to oil-derived plastics. However, PHAs are currently …

By leveraging advances in DNA synthesis and molecular cloning techniques, synthetic
biology increasingly makes use of large construct libraries to explore large design spaces. For …

Background The automation of modular cloning methodologies permits the assembly of many
genetic designs. Utilising characterised biological parts aids in the design and redesign of …

Natural products and their analogues are often challenging to synthesize due to their
complex scaffolds and embedded functional groups. Solely relying on engineering the …

Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a
perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to …

The use of self-correction strategy helps students to detect their own mistakes and self-correct
them, thereby students self-regulate their own learning processes. It is still a strategy that …

Indolocarbazoles are natural products with a broad spectrum of reported bioactivities. A
distinct feature of indolocarbazole biosynthesis is the modification of the indole and maleimide …

… Moore, Hung-En Lai, Richard JR Kelwick, Soo Mei Chee, David J. Bell, Karen Marie
Polizzi, and Paul S. Freemont* …