A tethered ligand assay to probe SARS-CoV-2: ACE2 interactions

MS Bauer, S Gruber, A Hausch… - Proceedings of the …, 2022 - National Acad Sciences
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections are initiated by
attachment of the receptor-binding domain (RBD) on the viral Spike protein to angiotensin-…

Multiplexed protein force spectroscopy reveals equilibrium protein folding dynamics and the low-force response of von Willebrand factor

…, PU Walker, SM Sedlak, S Gruber… - Proceedings of the …, 2019 - National Acad Sciences
Single-molecule force spectroscopy has provided unprecedented insights into protein
folding, force regulation, and function. So far, the field has relied primarily on atomic force …

Single-molecule force stability of the SARS-CoV-2–ACE2 interface in variants-of-concern

MS Bauer, S Gruber, A Hausch, MCR Melo… - Nature …, 2023 - nature.com
Mutations in SARS-CoV-2 have shown effective evasion of population immunity and increased
affinity to the cellular receptor angiotensin-converting enzyme 2 (ACE2). However, in the …

A conformational transition of the D′ D3 domain primes von Willebrand factor for multimerization

S Gruber, A Löf, A Hausch, F Kutzki, R Jöhr… - Blood …, 2022 - ashpublications.org
Von Willebrand factor (VWF) is a multimeric plasma glycoprotein that is critically involved in
hemostasis. Biosynthesis of long VWF concatemers in the endoplasmic reticulum and the …

Designed anchoring geometries determine lifetimes of biotin–streptavidin bonds under constant load and enable ultra-stable coupling

S Gruber, A Löf, SM Sedlak, M Benoit, HE Gaub… - Nanoscale, 2020 - pubs.rsc.org
The small molecule biotin and the homotetrameric protein streptavidin (SA) form a stable and
robust complex that plays a pivotal role in many biotechnological and medical applications. …

[PDF][PDF] The Low-Force Response of Von Willebrand Factor Revealed by Magnetic Tweezers

S Gruber, A Löf, T Obser, MA Brehm, M Benoit… - Biophysical …, 2020 - cell.com
Wednesday, February 19, 2020 619a the force range accessible by most methods of single-molecule
force spectroscopy. We established an assay based on magnetic tweezers that …

A tethered ligand assay to probe the SARS-CoV-2 ACE2 interaction under constant force

MS Bauer, S Gruber, LF Milles, T Nicolaus… - BioRxiv, 2020 - biorxiv.org
The current COVID-19 pandemic has a devastating global impact and is caused by the
SARS-CoV-2 virus. SARS-CoV-2 attaches to human host cells through interaction of its receptor …

A Conformational Transition of Von Willebrand Factor's D'D3 Domain Primes It For Multimerization

S Gruber, A Löf, A Hausch, R Jöhr, T Obser, G König… - bioRxiv, 2021 - biorxiv.org
Von Willebrand factor (VWF) is a multimeric plasma glycoprotein that is critically involved in
hemostasis. Biosynthesis of long VWF concatemers in the endoplasmic reticulum and the (…

[HTML][HTML] VR in Nursing Facilities-A randomized controlled multicenter pilot study analyzing the changes in the state of mind of seniors in nursing facilities through the …

SS Gruber, A Weigel, T Tischendorf, T Schaal… - Journal of Public …, 2022 - Springer
Context VR as an application to enhance well-being is sparsely researched in the elderly
population. The aim of the pilot study was to analyze the effect of 360 videos of different …

Effects of Variants of Concern Mutations on the Force-Stability of the SARS-CoV-2: ACE2 Interface and Virus Transmissibility

MS Bauer, S Gruber, A Hausch, MCR Melo… - bioRxiv, 2023 - biorxiv.org
Viruses mutate under a variety of selection pressures, allowing them to continuously adapt to
their hosts. Mutations in SARS-CoV-2 have shown effective evasion of population immunity …