Despite the efforts that bioengineers have exerted in designing and constructing biological
processes that function according to a predetermined set of rules, their operation remains …

We have used a human artificial chromosome (HAC) to manipulate the epigenetic state of
chromatin within an active kinetochore. The HAC has a dimeric α-satellite repeat containing …

Cleavage factor I m (CF I m ) is required for the first step in pre-mRNA 3′-end processing
and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. …

Survivin is a key cellular protein thought to function in apoptotic regulation, mitotic progression,
or possibly both. In this study, we describe the isolation of two conditional knockouts of …

Predictable operation of engineered biological circuitry requires the knowledge of host factors
that compete or interfere with designed function. Here, we perform a detailed analysis of …

We previously used a human artificial chromosome (HAC) with a synthetic kinetochore that
could be targeted with chromatin modifiers fused to tetracycline repressor to show that …

Mammalian cleavage factor I (CF I m ) is an essential factor that is required for the first step
in pre-mRNA 3′ end processing. Here, we characterize CF I m 68 subnuclear distribution …

Export of mRNA from the nucleus is linked to proper processing and packaging into
ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3…

Production of chemicals in microbes often employs potent biosynthetic enzymes, which can
interact with the microbial native metabolism to affect cell fitness and product yield. However, …

Cattle production is one of the key contributors to global warming due to methane emission,
which is a by-product of converting feed stuff into milk and meat for human consumption. …