Background Mapping of orthologous genes among species serves an important role in
functional genomics by allowing researchers to develop hypotheses about gene function in one …

To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi
Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: …

The ability to engineer genomes in a specific, systematic, and cost-effective way is critical
for functional genomic studies. Recent advances using the CRISPR-associated single-guide …

Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled
genome-wide functional screens in cultured cells and in vivo in model organisms. These screens …

10.7554/eLife.35574.001 We generated a library of ~1000 Drosophila stocks in which we
inserted a construct in the intron of genes allowing expression of GAL4 under control of …

Our understanding of the genetic mechanisms that underlie biological processes has relied
extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to …

One major challenge encountered with interpreting human genetic variants is the limited
understanding of the functional impact of genetic alterations on biological processes. …

Methionine restriction (MetR) extends lifespan across different species and exerts beneficial
effects on metabolic health and inflammatory responses. In contrast, certain cancer cells …

RNA interference (RNAi) leads to sequence‐specific knockdown of gene function. The approach
can be used in large‐scale screens to interrogate function in various model organisms …

In Drosophila melanogaster, formation of the axes and the primordial germ cells is regulated
by interactions between the germ line-derived oocyte and the surrounding somatic follicle …