[HTML][HTML] Single-cell copy number variant detection reveals the dynamics and diversity of adaptation
Copy number variants (CNVs) are a pervasive source of genetic variation and evolutionary
potential, but the dynamics and diversity of CNVs within evolving populations remain unclear. …
potential, but the dynamics and diversity of CNVs within evolving populations remain unclear. …
CREEPY: CRISPR-mediated editing of synthetic episomes in yeast
Use of synthetic genomics to design and build ‘big’ DNA has revolutionized our ability to
answer fundamental biological questions by employing a bottom-up approach. Saccharomyces …
answer fundamental biological questions by employing a bottom-up approach. Saccharomyces …
An evolving view of copy number variants
S Lauer, D Gresham - Current genetics, 2019 - Springer
Copy number variants (CNVs) are regions of the genome that vary in integer copy number.
CNVs, which comprise both amplifications and deletions of DNA sequence, have been …
CNVs, which comprise both amplifications and deletions of DNA sequence, have been …
[PDF][PDF] Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions
The Sc2.0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2.0 chromosomes assembled. Here, we describe …
has been achieved with all individual Sc2.0 chromosomes assembled. Here, we describe …
[PDF][PDF] Context-dependent neocentromere activity in synthetic yeast chromosome VIII
Pioneering advances in genome engineering, and specifically in genome writing, have
revolutionized the field of synthetic biology, propelling us toward the creation of synthetic …
revolutionized the field of synthetic biology, propelling us toward the creation of synthetic …
[PDF][PDF] Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics
We describe construction of the synthetic yeast chromosome XI (synXI) and reveal the effects
of redesign at non-coding DNA elements. The 660-kb synthetic yeast genome project (Sc2.…
of redesign at non-coding DNA elements. The 660-kb synthetic yeast genome project (Sc2.…
[PDF][PDF] Manipulating the 3D organization of the largest synthetic yeast chromosome
…, X Sun, Q Jiang, GW Goldberg, DM Ichikawa, SL Lauer… - Molecular Cell, 2023 - cell.com
Whether synthetic genomes can power life has attracted broad interest in the synthetic biology
field. Here, we report de novo synthesis of the largest eukaryotic chromosome thus far, …
field. Here, we report de novo synthesis of the largest eukaryotic chromosome thus far, …
[HTML][HTML] Spared piriform cortical single-unit odor processing and odor discrimination in the Tg2576 mouse model of Alzheimer's disease
…, M Lopez-Guzman, C Schoen, S Fitzgerald, SL Lauer… - PloS one, 2014 - journals.plos.org
Alzheimer's disease is a neurodegenerative disorder that is the most common cause of
dementia in the elderly today. One of the earliest reported signs of Alzheimer's disease is …
dementia in the elderly today. One of the earliest reported signs of Alzheimer's disease is …
[PDF][PDF] Synthetic chromosome fusion: Effects on mitotic and meiotic genome structure and function
We designed and synthesized synI, which is ∼21.6% shorter than native chrI, the smallest
chromosome in Saccharomyces cerevisiae. SynI was designed for attachment to another …
chromosome in Saccharomyces cerevisiae. SynI was designed for attachment to another …
Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand
We describe construction of the 660 kilobase synthetic yeast chromosome XI (synXI) and
reveal how synthetic redesign of non-coding DNA elements impact the cell. To aid construction …
reveal how synthetic redesign of non-coding DNA elements impact the cell. To aid construction …