Synthetic biology offers great promise to a variety of applications through the forward engineering
of biological function. Most efforts in this field have focused on employing living cells, …

Current programmable nuclease-based methods (for example, CRISPR–Cas9) for the precise
correction of a disease-causing genetic mutation harness the homology-directed repair …

Bacteria adapt to environmental stress by producing proteins that provide stress protection.
However, stress can severely perturb the kinetics of gene expression, disrupting protein …

While genome editing has been revolutionized by the advent of CRISPR-based nucleases,
difficulties in achieving efficient, nuclease-mediated, homology-directed repair (HDR) still …

Engineered gene circuits offer an opportunity to harness biological systems for biotechnological
and biomedical applications. However, reliance on native host promoters for the …

mRNA expression involves transcription initiation, elongation and degradation. In cells, these
dynamic processes are highly regulated. However, experimental characterization of the …

A fluorescent-probe-tumor-visualization platform was developed and validated in human
keratinocyte carcinoma excision specimens that may provide simple, rapid, and global …

Realizing the potential of cell-free systems will require development of ligand-sensitive
gene promoters that control gene expression in response to a ligand of interest. Here, we …

Neurofibromatosis type 1 (NF1), one of the most common autosomal dominant genetic
disorders, is caused by mutations in the NF1 gene. NF1 patients have a wide variety of …

In an effort to increase the efficiency and cure rate of nonmelanoma skin cancer (NMSC)
excisions, we have developed a point-of-care method of imaging and evaluation of skin cancer …