[HTML][HTML] Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection

SK Elledge, XX Zhou, JR Byrnes, AJ Martinko… - Nature …, 2021 - nature.com
Current serology tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
antibodies mainly take the form of enzyme-linked immunosorbent assays, chemiluminescent …

SARS-CoV-2 antibody magnitude and detectability are driven by disease severity, timing, and assay

…, C DiGermanio, M Stone, MP Busch, SK Elledge… - Science …, 2021 - science.org
Interpretation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
serosurveillance studies is limited by poorly defined performance of antibody assays over time in …

Nanoporous immunoprotective device for stem-cell-derived β-cell replacement therapy

…, SK Elledge, DA Bernards, JL Allen, K Villanueva… - ACS …, 2017 - ACS Publications
Encapsulation of human embryonic stem-cell-differentiated beta cell clusters (hES-βC) holds
great promise for cell replacement therapy for the treatment of diabetics without the need …

Competitive SARS-CoV-2 serology reveals most antibodies targeting the spike receptor-binding domain compete for ACE2 binding

JR Byrnes, XX Zhou, I Lui, SK Elledge, JE Glasgow… - MSphere, 2020 - Am Soc Microbiol
As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread
around the world, there is an urgent need for new assay formats to characterize the humoral …

[HTML][HTML] Evaluation of three morphologically distinct virus-like particles as nanocarriers for convection-enhanced drug delivery to glioblastoma

…, IL Aanei, JM Bernard, SH Klass, SK Elledge, K Han… - Nanomaterials, 2018 - mdpi.com
Glioblastoma is a particularly challenging cancer, as there are currently limited options for
treatment. New delivery routes are being explored, including direct intratumoral injection via …

Targeting and monitoring ovarian cancer invasion with an RNAi and peptide delivery system

L Hao, N Boehnke, SK Elledge… - Proceedings of the …, 2024 - National Acad Sciences
RNA interference (RNAi) therapeutics are an emerging class of medicines that selectively
target mRNA transcripts to silence protein production and combat disease. Despite the recent …

Systematic identification of engineered methionines and oxaziridines for efficient, stable, and site-specific antibody bioconjugation

SK Elledge, HL Tran, AH Christian… - Proceedings of the …, 2020 - National Acad Sciences
The field of chemical modification of proteins has been dominated by random modification of
lysines or more site-specific labeling of cysteines, each with attendant challenges. Recently…

Trimeric SARS-CoV-2 Spike interacts with dimeric ACE2 with limited intra-Spike avidity

I Lui, XX Zhou, SA Lim, SK Elledge, P Solomon… - BioRxiv, 2020 - biorxiv.org
A serious public health crisis is currently unfolding due to the SARS-CoV-2 pandemic. SARS-CoV-2
viral entry depends on an interaction between the receptor binding domain of the …

Bispecific VH/Fab antibodies targeting neutralizing and non-neutralizing Spike epitopes demonstrate enhanced potency against SARS-CoV-2

…, NJ Rettko, P Solomon, J Jin, I Lui, SK Elledge… - MAbs, 2021 - Taylor & Francis
Numerous neutralizing antibodies that target SARS-CoV-2 have been reported, and most
directly block binding of the viral Spike receptor-binding domain (RBD) to angiotensin-…

Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells

LL Kirkemo, SK Elledge, J Yang, JR Byrnes… - Elife, 2022 - elifesciences.org
Abstract Characterization of cell surface proteome differences between cancer and healthy
cells is a valuable approach for the identification of novel diagnostic and therapeutic targets. …