Highly sensitive techniques, such as PCR, have greatly improved the detection of respiratory
viruses. However, the sensitivity of PCR tests also complicates clinical interpretation, as the …

In 2005, a human bocavirus was discovered in children with respiratory tract illnesses.
Attempts to culture this virus on conventional cell lines has failed thus far. We investigated …

The human airway epithelium (HAE) represents the entry port of many human respiratory
viruses, including human coronaviruses (HCoVs). Nowadays, four HCoVs, HCoV-229E, HCoV-…

Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease, cirrhosis, and
hepatocellular carcinoma in humans and afflicts more than 58 million people worldwide. The …

Hepatitis C virus (HCV) infection affects approximately 58 million people and causes ~300,000
deaths yearly. The only target for HCV neutralizing antibodies is the highly sequence …

Hepatitis C virus (HCV) genotype 4 (HCV-4) infection is considered to be difficult to treat
and has become increasingly prevalent in European countries, including The Netherlands. …

In 5–40% of respiratory infections in children, the diagnostics remain negative, suggesting
that the patients might be infected with a yet unknown pathogen. Virus discovery cDNA-AFLP (…

Background Multiplex real time PCR is increasingly used to diagnose respiratory viruses
and has shown to be superior to traditional methods, like culture and antigen detection. …

Background Hepatitis C virus (HCV) is a rapidly evolving RNA virus that has been classified
into seven genotypes. All HCV genotypes cause chronic hepatitis, which ultimately leads to …

Since its initial identification in St. Petersburg, Russia, the recombinant hepatitis C virus (HCV)
2k/1b has been isolated from several countries throughout Eurasia. The 2k/1b strain is the …