A scalable transfection procedure using polyethylenimine (PEI) is described for the human
embryonic kidney 293 cell line grown in suspension. Green fluorescent protein (GFP) and …

Large‐scale transient transfection of mammalian cells is a recent and powerful technology
for the fast production of milligram amounts of recombinant proteins (r‐proteins). As many r‐…

Background Mammalian cells are becoming the prevailing expression system for the production
of recombinant proteins because of their capacity for proper protein folding, assembly, …

Respiration rates in Spodoptera frugiperda (Sf‐9) cell bioreactor cultures were successfully
measured on‐line using two methods: The O 2 uptake rate (OUR) was determined using gas …

Transient gene expression in mammalian cells is a valuable alternative to stable cell lines
for the rapid production of large amounts of recombinant proteins. While the establishment of …

We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled
receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein–Barr …

Recombinant forms of the spike protein of SARS-CoV-2 and related viruses have proven
difficult to produce with good yields in mammalian cells. Given the panoply of potential COVID-…

Nanobodies offer several potential advantages over mAbs for the control of SARS-CoV-2.
Their ability to access cryptic epitopes conserved across SARS-CoV-2 variants of concern (…

To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese
Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient …

Protein expression from stably transfected Chinese hamster ovary (CHO) clones is an
established but time‐consuming method for manufacturing therapeutic recombinant proteins. The …