User profiles for T. Huser

Thomas Huser

Professor of Physics, Bielefeld University
Verified email at physik.uni-bielefeld.de
Cited by 14568

Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
… For instance, Jung et al. used SMLM in combination with variable angle TIRF, scanning
and transmission EM to determine that T-cell receptors are highly localized on microvilli of T-cells…

Super-resolution structured illumination microscopy

R Heintzmann, T Huser - Chemical reviews, 2017 - ACS Publications
… , CellMask Orange (or its DeepRed variation), worked well for staining T cells and enabled
us to follow the transmission of HIV between T cells, (48) while the same stain had a rather …

Chemical analysis in vivo and in vitro by Raman spectroscopy—from single cells to humans

S Wachsmann-Hogiu, T Weeks, T Huser - Current opinion in biotechnology, 2009 - Elsevier
The gold standard for clinical diagnostics of tissues is immunofluorescence staining. Toxicity
of many fluorescent dyes precludes their application in vivo. Raman spectroscopy, a …

Surface-enhanced Raman scattering from individual Au nanoparticles and nanoparticle dimer substrates

…, NK Grady, CW Hollars, SM Lane, TR Huser… - Nano …, 2005 - ACS Publications
Surface-enhanced Raman scattering (SERS) intensities for individual Au nanospheres,
nanoshells, and nanosphere and nanoshell dimers coated with nonresonant molecules are …

[HTML][HTML] Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells

…, DS Taylor, T Zwerdling, SM Lane, K Ihara, T Huser - Biophysical journal, 2006 - cell.com
… In this analysis, 45 normal T-cell spectra were compared with 45 Jurkat T-cells. The
variance attributed to each of the first three PCs of the Raman spectra accounts for 51% of the …

Structural changes in fused silica after exposure to focused femtosecond laser pulses

JW Chan, T Huser, S Risbud, DM Krol - Optics letters, 2001 - opg.optica.org
… fictive temperature T f of the glass. One obtains glass with a high value of T f by fast quenching
from a high-temperature melt.[10] It is well known that for vitreous silica an increase in T f …

Unique gold nanoparticle aggregates as a highly active surface-enhanced Raman scattering substrate

…, CD Grant, A Wolcott, CE Talley, TR Huser… - The Journal of …, 2004 - ACS Publications
A unique gold nanoparticle aggregate (GNA) system has been shown to be an excellent
substrate for surface-enhanced Raman scattering (SERS) applications. Rhodamine 6G (R6G), …

Intracellular pH sensors based on surface-enhanced Raman scattering

…, L Jusinski, CW Hollars, SM Lane, T Huser - Analytical …, 2004 - ACS Publications
We present the development of nanoscale pH sensors based on functionalized silver
nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from …

Single chain spectroscopy of conformational dependence of conjugated polymer photophysics

T Huser, M Yan, LJ Rothberg - Proceedings of the National …, 2000 - National Acad Sciences
Single molecule confocal fluorescence microscopy was used to perform photoluminescence
spectroscopy on single, isolated molecules of the conjugated polymer poly[2-methoxy,5-(2…

[HTML][HTML] Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ

…, V Mönkemöller, S Hennig, W Hübner, T Huser - Nature …, 2016 - nature.com
Super-resolved structured illumination microscopy (SR-SIM) is an important tool for
fluorescence microscopy. SR-SIM microscopes perform multiple image acquisitions with varying …