Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets.
Such "onco-lncRNAs" have resisted traditional RNAi methods, but CRISPR-Cas9 genome …

CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of
single guide RNAs (sgRNAs). This approach has been widely adopted for diverse …

The sequence domains underlying long noncoding RNA (lncRNA) activities, including their
characteristic nuclear enrichment, remain largely unknown. It has been proposed that these …

Long noncoding RNAs (lncRNAs) are widely dysregulated in cancer, yet their functional roles
in cancer hallmarks remain unclear. We employ pooled CRISPR deletion to perturb 831 …

Background Danon disease (DD) is a rare disorder characterized by cardiomyopathy,
intellectual disability, and proximal myopathy. It is caused by mutations in the LAMP2 gene on X …

Long noncoding RNAs (lncRNAs) are linked to cancer via pathogenic changes in their
expression levels. Yet, it remains unclear whether lncRNAs can also impact tumour cell fitness via …

A key attribute of some long noncoding RNAs (lncRNAs) is their ability to regulate expression
of neighbouring genes in cis. However, such ‘cis-lncRNAs’ are presently defined using ad …

Long noncoding RNAs (lncRNAs) are believed to regulate expression of neighbouring target
genes. However, such ‘cis-lncRNAs’ are presently defined using ad hoc criteria that, we …

Using CRISPR/Cas9, diverse genomic elements may be studied in their endogenous context.
Pairs of single guide RNAs (sgRNAs) are used to delete regulatory elements and small …

Long noncoding RNAs (lncRNAs) can positively and negatively regulate expression of target
genes encoded in cis. However, the extent, characteristics and mechanisms of such cis- …