The Sam68 nuclear body is composed of two RNase-sensitive substructures joined by the adaptor HNRNPL

T Mannen, S Yamashita, K Tomita, N Goshima… - Journal of cell …, 2016 - rupress.org
The mammalian cell nucleus contains membraneless suborganelles referred to as nuclear
bodies (NBs). Some NBs are formed with an architectural RNA (arcRNA) as the structural core…

Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles

S Hennig, G Kong, T Mannen, A Sadowska… - Journal of Cell …, 2015 - rupress.org
Prion-like domains (PLDs) are low complexity sequences found in RNA binding proteins
associated with the neurodegenerative disorder amyotrophic lateral sclerosis. Recently, PLDs …

Distinct RNA polymerase transcripts direct the assembly of phase-separated DBC1 nuclear bodies in different cell lines

T Mannen, M Goto, T Yoshizawa… - Molecular Biology of …, 2021 - Am Soc Cell Biol
The mammalian cell nucleus is a highly organized organelle that contains membrane-less
structures referred to as nuclear bodies (NBs). Some NBs carry specific RNA types that play …

[HTML][HTML] Mechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS (P525L) and FUS (R495X)

A Gonzalez, T Mannen, T Çağatay, A Fujiwara… - Scientific reports, 2021 - nature.com
Mutations in the RNA-binding protein FUS cause familial amyotropic lateral sclerosis (ALS).
Several mutations that affect the proline-tyrosine nuclear localization signal (PY-NLS) of …

[HTML][HTML] C9orf72-derived arginine-rich poly-dipeptides impede phase modifiers

…, M Matsubayashi, Y Shinkai, T Niwa, T Mannen… - Nature …, 2021 - nature.com
Nuclear import receptors (NIRs) not only transport RNA-binding proteins (RBPs) but also
modify phase transitions of RBPs by recognizing nuclear localization signals (NLSs). Toxic …

How does the royal family of Tudor rule the PIWI-interacting RNA pathway?

MC Siomi, T Mannen, H Siomi - Genes & development, 2010 - genesdev.cshlp.org
PIWI (P-element-induced wimpy testis) proteins are a subset of the Argonaute proteins and
are expressed predominantly in the germlines of a variety of organisms, including Drosophila …

[PDF][PDF] Respective functions of two distinct Siwi complexes assembled during PIWI-interacting RNA biogenesis in Bombyx germ cells

…, YW Iwasaki, Y Murota, A Nagao, T Mannen… - Cell reports, 2015 - cell.com
PIWI-interacting RNA (piRNA) biogenesis consists of two sequential steps: primary piRNA
processing and the ping-pong cycle that depends on reciprocal Slicer-mediated RNA …

Dss1 associating with the proteasome functions in selective nuclear mRNA export in yeast

T Mannen, T Andoh, T Tani - Biochemical and biophysical research …, 2008 - Elsevier
Dss1p is an evolutionarily conserved small protein that interacts with BRCA2, a tumor suppressor
protein, in humans. The Schizosaccharomyces pombe strain lacking the dss1 + gene (…

Siwi levels reversibly regulate secondary piRISC biogenesis by affecting Ago3 body morphology in Bombyx mori

…, T Sumiyoshi, H Yamazaki, T Mannen… - The EMBO …, 2020 - embopress.org
Silkworm ovarian germ cells produce the Siwi‐pi RNA ‐induced silencing complex (pi RISC
) through two consecutive mechanisms, the primary pathway and the secondary ping‐pong …

ArcRNAs and the formation of nuclear bodies

S Nakagawa, T Yamazaki, T Mannen, T Hirose - Mammalian Genome, 2022 - Springer
Long noncoding RNAs (lncRNAs) have long been collectively and passively defined as
transcripts that do not encode proteins. However, extensive functional studies performed over …