Many chromatin features play critical roles in regulating gene expression. A complete
understanding of gene regulation will require the mapping of specific chromatin features in small …

To gain insight into how genomic information is translated into cellular and developmental
programs, the Drosophila model organism Encyclopedia of DNA Elements (modENCODE) …

10.7554/eLife.46314.001 Previously, we described a novel alternative to chromatin
immunoprecipitation, CUT&RUN, in which unfixed permeabilized cells are incubated with antibody, …

Background Centromeres represent the last frontiers of plant and animal genomics. Although
they perform a conserved function in chromosome segregation, centromeres are typically …

We have developed a chemical cleavage method that allows precise mapping of both single
nucleosomes and linkers genome-wide in budding yeast. We confirm that S. cerevisiae …

Micrococcal nuclease (MNase) is widely used to map nucleosomes. However, its aggressive
endo-/exo-nuclease activities make MNase-seq unreliable for determining nucleosome …

10.7554/eLife.01861.001 In budding yeast, a single cenH3 (Cse4) nucleosome occupies the
∼120-bp functional centromere, however conflicting structural models for the particle have …

Background DNA methylation occurs at preferred sites in eukaryotes. In Arabidopsis, DNA
cytosine methylation is maintained by three subfamilies of methyltransferases with distinct …

The two doublet histones of Marseillevirus are distantly related to the four eukaryotic core
histones and wrap 121 base pairs of DNA to form remarkably similar nucleosomes. By …

The expression and genome-wide mapping of epitope-tagged DNA- and chromatin-binding
proteins in cultured cells has become a powerful strategy for epigenome characterization, …