Protein interaction networks and protein compartmentalization underlie all signaling and
regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new …

This protocol describes the use of TurboID and split-TurboID in proximity labeling applications
for mapping protein–protein interactions and subcellular proteomes in live mammalian …

Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the
proteomic analysis of subcellular regions difficult or impossible to access by conventional …

Defining specific protein interactions and spatially or temporally restricted local proteomes
improves our understanding of all cellular processes, but obtaining such data is challenging, …

Nucleotide-binding leucine-rich repeat (NLR) immune receptors play a critical role in
defence against pathogens in plants and animals. However, we know very little about NLR-…

Microtubules are polarized intracellular polymers that play key roles in the cell, including in
transport, polarity, and cell division. Across eukaryotic cell types, microtubules adopt diverse …

Proteome profiling is a powerful tool in biological and biomedical studies, starting with samples
at bulk, single-cell, or single-cell-type levels. Reliable methods for extracting specific cell-…

Toxoplasma gondii is a ubiquitous, intracellular parasite that envelops its parasitophorous
vacuole with a protein-laden membrane (PVM). The PVM is critical for interactions with the …

Primary cilia are sensory organelles crucial for cell signaling during development and organ
homeostasis. Cilia arise from centrosomes and their formation and function is governed by …

… Tess C Branon Tess C Branon … Data was analyzed by calculating dC t values, in which the
C t of the housekeeping gene was subtracted from the C t of the gene of interest. Then ddC t …