In mammalian cells, >25% of synthesized proteins are exported through the secretory
pathway. The pathway complexity, however, obfuscates its impact on the secretion of different …

Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for production
of therapeutic proteins. However, development of recombinant CHO cell lines has been …

Chinese hamster ovary (CHO) cells are widely used in the biopharmaceutical industry as a
host for the production of complex pharmaceutical proteins. Thus genome engineering of …

The CRISPR/Cas9 genome editing technology has previously been shown to be a highly
efficient tool for generating gene disruptions in CHO cells. In this study we further demonstrate …

Recombinant protein production coopts the host cell machinery to provide high protein yields
of industrial enzymes or biotherapeutics. However, since protein translation is energetically …

A large part of our current understanding of gene regulation in Gram-positive bacteria is
based on Bacillus subtilis , as it is one of the most well studied bacterial model systems. The …

… We demonstrate that the disruption of flagella in a B. subtilis α-amylase production strain,
either by CRISPR-dCas9-based knockdown of the operon or by replacing flgE with an …

Yield improvements in cell factories can potentially be obtained by fine-tuning the regulatory
mechanisms for gene candidates. In pursuit of such candidates, we performed RNA-…

In S. cerevisiae cells debilitated in mRNA nuclear export, transcripts are retained in nuclear
foci ("dots"). The ultimate fate of dot-mRNA has remained elusive. Here, we use single …

Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has
traditionally been performed with ELISA or HPLC. As these techniques suffer from several …