Background High throughput sequencing has become an important technology for studying
expression levels in many types of genomic, and particularly transcriptomic, data. One key …

A silencing signal in plants with an RNA specificity determinant moves through plasmodesmata
and the phloem. To identify the mobile RNA, we grafted Arabidopsis thaliana shoots to …

Argonaute (AGO) effectors of RNA silencing bind small RNA (sRNA) molecules and
mediate mRNA cleavage, translational repression, or epigenetic DNA modification. In many …

PRDM9 directs human meiotic crossover hot spots to intergenic sequence motifs, whereas
budding yeast hot spots overlap regions of low nucleosome density (LND) in gene promoters. …

RNA silencing at the transcriptional and posttranscriptional levels regulates endogenous
gene expression, controls invading transposable elements (TEs), and protects the cell against …

Meiotic recombination initiates from DNA double-strand breaks (DSBs) generated by SPO11
topoisomerase-like complexes. Meiotic DSB frequency varies extensively along eukaryotic …

During meiosis, homologous chromosomes undergo crossover recombination, which is
typically concentrated in narrow hot spots that are controlled by genetic and epigenetic …

The ability for cut tissues to join and form a chimeric organism is a remarkable property of
many plants; however, grafting is poorly characterized at the molecular level. To better …

Ribosome profiling is a technique that permits genome-wide, quantitative analysis of translation
and has found broad application in recent years. Here we describe a modified profiling …

Meiotic crossover frequency varies extensively along chromosomes and is typically
concentrated in hotspots. As recombination increases genetic diversity, hotspots are predicted to …