The Golgi-associated protein ArfGAP1 has an unusual membrane-adsorbing amphipathic α-helix:
its polar face is weakly charged, containing mainly serine and threonine residues. We …

Eukaryotic cells coordinate growth with the availability of nutrients through the mechanistic
target of rapamycin complex 1 (mTORC1), a master growth regulator. Leucine is of particular …

Linker of nucleoskeleton and cytoskeleton (LINC) complexes span the nuclear envelope
and are composed of KASH and SUN proteins residing in the outer and inner nuclear …

Nuclear pore complexes (NPCs) perforate the nuclear envelope and serve as the primary
transport gates for molecular exchange between nucleus and cytoplasm. Stripping the …

Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE) 1 , 2 – 3 . These multi-megadalton …

The mechanistic Target of Rapamycin Complex 1 (mTORC1) is a major regulator of eukaryotic
growth that coordinates anabolic and catabolic cellular processes with inputs such as …

His‐tag affinity purification is one of the most commonly used methods to purify recombinant
proteins expressed in E. coli. One drawback of using the His‐tag is the co‐purification of …

Nuclear pore complexes (NPCs) are indispensable for cell function and are at the center of
several human diseases. NPCs provide access to the nucleus and regulate the transport of …

Nuclear pore complexes (NPCs) facilitate nucleocytoplasmic transport. These massive
assemblies comprise an eightfold symmetric scaffold of architectural proteins and central-channel …

Nuclear pore complexes (NPCs) perforate the nuclear envelope and represent the exclusive
passageway into and out of the nucleus of the eukaryotic cell. Apart from their essential …