In eukaryotic cells, post-translational histone modifications have an important role in gene
regulation. Starting with early work on histone acetylation 1 , a variety of residue-specific …

Although messenger RNA (mRNA) translation is a fundamental biological process, it has
never been imaged in real time in vivo with single-molecule precision. To achieve this, we …

Ribonucleoprotein (RNP) granules are non-membrane-bound organelles that have critical
roles in the stress response 1 , 2 , maternal messenger RNA storage 3 , synaptic plasticity 4 , …

Histone modifications play an important role in epigenetic gene regulation and genome
integrity. It remains largely unknown, however, how these modifications dynamically change in …

We describe an engineered family of highly antigenic molecules based on GFP-like
fluorescent proteins. These molecules contain numerous copies of peptide epitopes and …

The binding of nuclear proteins to chromatin in live cells has been analyzed by kinetic
modeling procedures applied to experimental data from fluorescence recovery after …

Measurement of live-cell binding interactions is vital for understanding the biochemical
reactions that drive cellular processes. Here, we develop, characterize, and apply a new …

For gene regulation, some transcriptional activators bind periodically to promoters with either
a fast (∼1 minute) or a slow (∼15 to 90 minutes) cycle. It is uncertain whether the fast cycle …

Post-translational histone modifications play key roles in gene regulation, development and
differentiation, but their dynamics in living organisms remain almost completely unknown. To …

To expand the toolbox of imaging in living cells, we have engineered a single-chain variable
fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting …