Small molecules that perturb specific protein functions are valuable tools for dissecting
complex processes in mammalian cells. A combination of two phenotype-based screens, one …

We have developed a method to detect DNA synthesis in proliferating cells, based on the
incorporation of 5-ethynyl-2′-deoxyuridine (EdU) and its subsequent detection by a …

Completion of cell division during cytokinesis requires temporally and spatially regulated
communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell …

The dynamic behaviour of actin filaments has been directly observed in living, motile cells
using fluorescence photoactivation. In goldfish epithelial keratocytes, the actin microfilaments …

▪ Abstract The polymerization dynamics of microtubules are central to their biological
functions. Polymerization dynamics allow microtubules to adopt spatial arrangements that can …

The mechanism of apoptosis has been extensively characterized over the past decade, but
little is known about alternative forms of regulated cell death. Although stimulation of the Fas/…

Barrier structures (for example, epithelia around tissues and plasma membranes around
cells) are required for internal homeostasis and protection from pathogens. Wound detection …

For most intracellular structures with larger than molecular dimensions, little is known about
the connection between underlying molecular activities and higher order organization such …

In recent years the kinesin superfamily has become so large that several different naming
schemes have emerged, leading to confusion and miscommunication. Here, we set forth a …

Like neutrophilic leukocytes, differentiated HL-60 cells respond to chemoattractant by adopting
a polarized morphology, with F-actin in a protruding pseudopod at the leading edge and …